目的 研究斑唇马先蒿的化学成分及其抗氧化活性。方法 采用正相硅胶色谱柱、反相C18硅胶色谱柱、大孔树脂和高效液相等柱色谱分析手段,对藏药斑唇马先蒿的化学成分进行系统的分离纯化,并通过清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基的能力考察了甲醇粗提物、洗脱组分和化合物6~10的抗氧化活性。结果 得到10个化合物,根据化合物的波谱数据和理化性质分别将其鉴定为去氧土大黄苷[(E)-3-(β-D-glucopyranosyloxy)-5-hydroxy-4′-methoxystilbene](1)、β-谷甾醇(β-sitosterol)(2)、鸢尾葡萄糖苷(iridotrial glucoside)(3)、木犀草素(luteolin)(4)、苜蓿素(5,7,4′-trihydroxy-3′,5′-dimethoxyflavone)(5)、松果菊苷(echinacoside)(6)、焦地黄苯乙醇苷A1(jionoside A1)(7)、甘蓝苷H1(kankanoside H1)(8)、甘蓝苷l(kankanoside l)(9)和马先蒿苷Ⅰ(pedieularioside Ⅰ)(10)。另外,甲醇洗脱组分和化合物6的抗氧化活性较好。结论 化合物1, 3和6~10为首次从该植物中分到。
Abstract
OBJECTIVE To investigate the chemical constituents of Pedicularis longiflora Rudolph. var. tubiformis (Klotz). Tsoong systematically. METHODS Silica-gel column, reversed phase C18 column, macroporous resin column and Sephadex LH-20 column were employed to purify the chemical constituents. The ability to remove DPPH free radicals was determined to evaluate the antioxidant activities of the crude extract, eluted fractions and compounds. RESULTS Ten compounds were obtained and identified as (E)-3-(β-D-glucopyranosyloxy)-5-hydroxy-4′-methoxystilbene (1), β-sitosterol (2), iridotrial glucoside (3), luteolin(4), 5,7,4′-trihydroxy-3′,5′-dimethoxyflavone (5), echinacoside (6), jionoside A1 (7), kankanoside H1 (8), kankanoside l (9) and pedieularioside I (10) based on the NMR spectra together with physical and chemical properties. In addition, the methanol-eluted fractions and compound 6 showed moderate antioxidant activity. CONCLUSION Compounds 1, 3 and 6-10 are isolated form Pedicularis longiflora Rudolph.var. tubiformis (Klotz).Tsoong for the first time.
关键词
斑唇马先蒿 /
1 /
1-二苯基-2-三硝基苯肼(DPPH) /
抗氧化活性 /
松果菊苷 /
焦地黄苯乙醇苷A1 /
甘蓝苷H1
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Key words
Pedicularis longiflora Rudolph.var. tubiformis (Klotz).Tsoong /
DPPH /
antioxidant activity /
echinacoside /
jionoside A1 /
kankanoside H1
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中图分类号：
R284
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参考文献
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脚注
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基金
重庆市基本科研业务费资助(16438);重庆市农业发展资金资助(17412)
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